The Most Comprehensive Source of Stereology Information on the Web


What is, the Stereology Information Center, includes a wide range of knowledge about stereology. This site introduces both basic and advanced concepts in the field. The emphasis is on its use in biological research, though anyone interested in learning concepts of stereology will find something of interest. The application of stereological methods to biological studies permits researchers to effectively and efficiently gather data. This site is dedicated to helping researchers understand the principles of design-based stereology and its advantages over less sophisticated approaches in quantitative histology.

Design-based stereology is a set of methods to ensure rigorous quantitative analysis of the size, shape, and number of objects. When properly used, stereology plays an important role in validating and rejecting experimental hypotheses in biological research. It produces results that are unbiased, efficient, and more reliable than other ad hoc quantitative analyses. Unbiased stereology provides an important contribution to the advancement in biological research by improving the consistency and dependability of quantitative analytical results produced in the laboratory and reported in scientific publications.

The Counting Frame is a fundamental tool in stereological research.

Recent Noteworthy Stereology Publications

Published papers using Stereology; last updated on 04/18/2014, * indicates an example where a good job was done reporting stereological parameters

nNOS inhibition during profound asphyxia reduces seizure burden and improves survival of striatal phenotypic neurons in preterm fetal sheep

Motor Impairments, Striatal Degeneration, and Altered Dopamine-Glutamate Interplay in Mice Lacking PSD-95

Patrolling monocytes play a critical role in CX3CR1-mediated neuroprotection during excitotoxicity

Spatial Working Memory Deficits in Male Rats Following Neonatal Hypoxic Ischemic Brain Injury Can Be Attenuatedby Task Modifications*

A Major External Source of Cholinergic Innervation of the Striatum and Nucleus Accumbens Originates in the Brainstem

Effects of high fat diet induced obesity on peripheral nerve regeneration and levels of GAP 43 and TGF-β in rats

Enhanced Adult Neurogenesis Increases Brain Stiffness: In Vivo Magnetic Resonance Elastography in a Mouse Model of Dopamine Depletion*

Glatiramer acetate reverses cognitive deficits from cranial-irradiated rat by inducing hippocampal neurogenesis

MMP-9 Inhibitor SB-3CT Attenuates Behavioral Impairments and Hippocampal Loss after Traumatic Brain Injury in Rat

Efficient specification of interneurons from human pluripotent stem cells by dorsoventral and rostrocaudal modulation

Do American Goldfinches See Their World Like Passive Prey Foragers A Study on Visual Fields, Retinal Topography, and Sensitivity of Photoreceptors

Abnormal vibrissa-related behavior and loss of barrel field inhibitory neurons in 5xFAD transgenics

Increased 18F-FDG Uptake Is Predictive of Rupture in a Novel Rat Abdominal Aortic Aneurysm Rupture Model

Extended Wakefulness: Compromised Metabolics in and Degeneration of Locus Ceruleus Neurons

Chemosensory cues affect amygdaloid neurogenesis and alter behaviors in the socially monogamous prairie vole

7,8-Dihydroxyflavone improves motor performance and enhances lower motor neuronal survival in a mouse model of amyotrophic lateral sclerosis

Astrocytic JWA Expression is Essential to Dopaminergic Neuron Survival in the Pathogenesis of Parkinson’s Disease

Protective effects of alpha-lipoic acid on experimental sciatic nerve crush injury in rats: assessed with functional, molecular and electromicroscopic analyses

High-Frequency Stimulation of the Subthalamic Nucleus Counteracts Cortical Expression of Major Histocompatibility Complex Genes in a Rat Model of Parkinson’s Disease

Neonatal Peripheral Immune Challenge Activates Microglia and Inhibits Neurogenesis in the Developing Murine Hippocampus

Combination Therapy of Human Umbilical Cord Blood Cells and Granulocyte Colony Stimulating Factor Reduces Histopathological and Motor Impairments in an Experimental Model of Chronic Traumatic Brain Injury

Subthalamic nucleus lesion improves cell survival and functional recovery following dopaminergic cell transplantation in parkinsonian rats

Aggrecan, link protein and tenascin-R are essential components of the perineuronal net to protect neurons against iron-induced oxidative stress

Brain-region-specific alterations of the trajectories of neuronal volume growth throughout the lifespan in autism

Iso-luminance counterillumination drove bioluminescent shark radiation

Human neural progenitor cells generated from induced pluripotent stem cells can survive, migrate, and integrate in the rodent spinal cord

α-Synuclein impairs oligodendrocyte progenitor maturation in multiple system atrophy

Neuronopathy in the motor neocortex in a chronic model of multiple sclerosis.

The effects of pre- and post-natal nicotine exposure and genetic background on the striatum and behavioral phenotypes in the mouse

Differential Neuroprotective Effects of 5′-Deoxy-5′-Methylthioadenosine*

Increased tyrosine hydroxylase expression accompanied by glial changes within the non-lesioned hemisphere in the 6-hydroxydopamine model of Parkinson’s disease

Implications of p75NTR for dentate gyrus morphology and hippocampus-related behavior revisited

No changes in cerebellar microvessel length density in sudden infant death syndrome: implications for pathogenetic mechanisms.

Cognitive Ecology in Hummingbirds: The Role of Sexual Dimorphism and Its Anatomical Correlates on Memory

Parvalbumin immunoreactivity and expression of GABAA receptor subunits in the thalamus after experimental TBI

Respiratory chain deficiency in aged spinal motor neurons

Tetanus toxin preserves skeletal muscle contractile force and size during limb immobilization

Gene Expression Alterations in the Cerebellum and Granule Neurons of Cstb−/− Mouse Are Associated with Early Synaptic Changes and Inflammation

Recommended Stereology Workshops and Courses


Organized by Dr. Daniel Peterson
August 18-22, 2014
Chicago, IL (USA)

Practical Workshops in Confocal Microscopy and Design-Based Stereology

Short Description
The purpose of this intensive, week-long workshop is to provide a comprehensive background in the theory and practice of modern histological preparation and microscopic analysis for researchers in biomedical science. The workshop provides a practical tutorial in all aspects of microscopic analysis including experimental design, specimen preparation, use of fluorescence and confocal microscopes, design-based stereology, and image preparation for scientific publication. A variety of microscopes equipped for stereology are available for use. Participants should bring examples of their material and problems for discussion. In addition to lectures, substantial time is devoted to “hands-on” practical sessions. Attendees participate in group discussion, where they can discuss their research applications and consult with the instructors.


Organized by Professor Mark West
November 13-14, 2014
The Westin Washington, D.C. City Center
Washington, D.C. (USA)

NeuroStereology Workshop

Short Description
The goal of the workshop is to teach a small group of research scientists how to design, supervise, and critically evaluate stereological studies of the nervous system. Stereology is a methodology that provides meaningful quantitative descriptions of the geometry of three-dimensional structures from measurements that are made on two-dimensional images sampled from a structure of interest. The workshop will emphasize modern ”design based” stereology, which is characterized by the preparation and probing of tissue in such a manner that no assumptions about the size, shape, and orientation of the structures being quantified are necessary.

The target audience includes neuroscience research scientists working at the graduate student, post-doctoral, and senior scientists who are interested in the practical application of design based stereological methods. The number of participants will be limited to 30, to ensure robust faculty-student interaction.


NotePlease note that MBF Bioscience only recommends courses and workshops taught by capable and qualified instructors with no commercial affiliations.