Have you prepared the tissue for visualization under the microscope?

IF NOT:

→ Here are some guidelines:

• Use systematic random sampling through the entire region of interest.
• Preferential sections are acceptable.
• Thick sections are preferable for use with the optical disector or optical fractionator probe.
• If it is not possible to obtain thick sections, use contiguous pairs of thin sections for the physical disector or physical fractionator probe.
• If the particles whose number you want to estimate are in a monolayer, for instance in a petri-dish or 2-D whole mount, use the disector or fractionator probe.
• For particles with dimples or bridges, e.g., alveoli in lungs, use the connectivity probe.